The larger segment, A, encodes VP2, VP4 and VP3 in large Open Reading Frame (ORF). The double stranded RNA genome of IBDV is composed of 2 segments A and B codes for five viral proteins (VP). The indirect economic impacts is due to IBDV-induced immunosuppression of infected birds, Infectious bursal disease viruses are non-enveloped, icosahydral member of the genus Avibirnaviridae. The direct economic impact of IBDV is due to the high mortality rates. Infectious Bursal Disease (IBD) serotype 1 viruses continue to cause direct and indirect significant economic losses to poultry industry. Keywords: Infectious Bursal Disease, Very Virulent, Vp2, Primer, Open Reading Frame (ORF), Reverse Transcription, Polymerase Chain Reaction, Sequence, Phylogenetic Analysis. This finding explains the continued presence of vvIBDV in vaccinated broiler flocks. Some of the amino acid mutations have lead to a change in some of the exposed regions of the viral protein. Phylogenetic analysis of five sequenced PCR products selected according to their superior amount of DNA contents and deduced amino acid sequences of IBDV-VP2 demonstrated the continued circulation of very virulent IBDV (vvIBDV). Infectious Bursal Disease virus was detected in all tested samples. Bursa samples were RT-PCR tested using novel primer flanking VP2 region coding the two major and two minor hydrophilic peaks. A recent increase in IBDV related mortality in vaccinated farms prompted this investigation of the genetic characterization of the circulating IBDVs. Ten of IBDV were selected from an infected commercial broiler flocks. ![]() This study was carried out to investigate the molecular characterization of the field Infectious Bursal Disease viruses (IBDVs) at Delta Governorates in Egypt.
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